Purifying proteins with Ni affinity chromatography and troubleshooting CHAPTERS: 00:00 Introduction 00:17 Make Buffers 01:43 The Strep-tag® Technology for Proteins
The structure of the SBP-Tag-streptavidin complex. Learn what Ni-NTA bead volume is required to purify your His-tagged protein in this short video. How to purify Proteins using Batch Spin
The Strep-tag® System: One Tag - Multiple Applications Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared
In affinity chromatography, the proteins you want selectively stick to the resin, while the proteins you don't want flow through. Strep-Tactin® vs Strep-Tactin®XT: comparing binding capacities IBA Lifesciences Strep-Tactin XT 4Flow Resin 20 mL | Buy Online
Strep-Tactin XT 4Flow 50% suspension is coupled to the high affinity ligand Strep-Tactin XT and can be used for the purific Accelerated Label-free Kinetics Screening in Early Single Domain Antibody Discovery VDS NiNTA columns
The Strep-tag® protein purification system comprises two affinity tags: the Strep-tag®II and its tandem version Twin-Strep-tag®. In this video Steven Bye, Marketing Manager of Roche Applied Science, discusses the cOmplete His-Tag
Streptavadin has a truly spectacular ability to bind biotin (one of the strongest “weak” (i.e. non-covalent) interactions known) and Automated transient protein expression— Expi293™ system on the ambr™ system Strep-Tactin XT Sepharose is a chromatography resin for purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®
Cube Biotech offers a high-quality affinity resin for strep-tagged proteins, with maximum binding capacity. Choose your preferred method from either agarose Protein Purification with Strep-Tactin®XT Spin Columns Strep-tag affinity chromatography (a protein purification method)
Let us show you how to pack an empty cartridge / column with one of our protein purification resins. The column / cartridge About IBA Lifesciences Biotinylation du réactif de capture
Presented At: BioProcessing Virtual Event 2019 Presented By: Dennis Karthaus, MS - Scientist and Team Leader, Cell Cultivation, "Accelerated Label-free Kinetics Screening in Early Single Domain Antibody Discovery: A Comparative Analysis with Phage The Strep-tag® system is one of the most widely used affinity systems for protein purification. Watch this webinar to get an insight
Investigating Protein-Protein Interactions with Strep-TactinXT with Christel Schmiechen GoBio Mini Columns Ni Protein Purification -Hancock Lab HD 1080p
Gyrolab® immunoassay solutions – Quatre étapes faciles pour coupler à la biotine et stocker le réactif de capture afin de Strep tag protein Strep-Tactin XT 4Flow high capacity is perfectly suited for purifying Strep-tag II or Twin-Strep-tag fusion proteins.
Strep-Tactin XT is the high affinity variant for the purification of strep-tagged fusion proteins, providing binding affinities in the picomolar range for Twin This video demonstrates how to fit an Aurora Series column to your UHPLC system for Thermo Scientific Nanospray Flex source
Label-free Cell Isolation from Blood with Strep-Tactin® TACS Agarose Protein desalting on a reversed phase for ESI-MS analysis under denaturing conditions.
Overcoming Limitations of Conventional Tag Systems - Strep-Tactin® XT Applications Strep-Tactin® and Strep-Tactin®XT resins are used for protein purification of recombinant Strep-tag®II or Twin-Strep-tag® CaptureSelect Technology - Powerful Affinity for More Streamlined Purification
Fab-TACS® is based on the IBA's universal Strep-tag® technology and allows label-free cell isolation from different samples via Streptactin Products | Cube Biotech Strep-Tactin®XT 4Flow® resin | 50 ml | 2-5010-025
This video was made by group 2 for group 9. Enjoy~!! Protein Purification I Ni NTA Column I Protein I procedure for protein purification. IBA Lifesciences GmbH is a biotechnology company providing products for life science research in academia and industry.
The Strep-tag® technology allows efficient one-step purification of Strep-tag®II or Twin-Strep-tag® proteins via affinity Purifying hybridoma IgG using a protein G column.
Strep-Tactin®XT has a higher binding affinity for Strep-tag®II and Twin-Strep-tag® compared to Strep-Tactin®. This feature allows Wet Fred is an application aid for purification of (Twin-)Strep-tag® fusion proteins via gravity flow columns containing
Video abstract of Review paper “Technology advancements in antibody purification” published in the open access journal Wet Fred is a flow regulation device and an application aid for purification of (Twin-)Strep-tag® fusion proteins. It enables the
Strep-Tactin™ XT Sepharose™ is a chromatography resin for purifying recombinant proteins with Strep-tag™ II or Twin-Strep-tag. Purification. Quick Tips Binding Capacity of Ni-NTA Magnetic Beads Fitting Aurora Series columns to Thermo Scientific Nanospray Flex source using Sonation Column Oven
Gator Bio Strep-Tactin XT probe is coated with Strep-Tactin XT that binds Twin-Strep-tag with pM affinity making it an ideal tool for quantitation and Efficient purification of recombinant Strep-tag® fusion proteins from Expi supernatants using..
IBA's unique Strep-tag® technology is a commonly used tool for the affinity purification of recombinant proteins and is based on Recom Purif Pt3 Purification of His-Tagged Protein Tutorial
Protein Purification I Ni NTA Column I Protein I procedure for protein purification Matching upstream and downstream processing is highly beneficial as it saves time and money in the process development and Intelligent Infusions Masterclass on Protein-Protein Interaction Workflows - April 2025 KEY TAKEAWAYS • Protein-protein
Protein Purification: Benefits of Strep-tag® system III Here I use an AKTA system to purify immunoglobulin from tissue culture supernatant on a protein G column. The technique avoids Learn how the breakthrough Expi293 Expression System can be combined with the TAP Biosystems ambr™ Advanced Micro
Connecting a Strep-Tactin® FPLC Column to an Äkta® Device Protein desalting - denaturing conditions
Protein Purification Tutorial with Strep-Tactin®XT 4Flow® I have been attempting to purify a recombinant protein fused at the C-terminus with Twin-StrepTag. For that I am using the Strep-Tactin XT 4Flow High Capacity IBA Lifesciences Strep-Tactin XT 4Flow Resin from IBA Lifesciences. Fisher Scientific - Strep-Tactin™XT 4Flow™ resin is a 50% suspension to purify
Strep-Tactin Tags for Nanobody Purification and Biolayer Interferometry Analysis Strep-Tactin®XT high capacity
cOmplete His-Tag Purification Resin from Roche Applied Science Protein Purification with Strep-Tactin®XT coated magnetic beads
Label-free Cell Isolation from Splenocytes with Strep-Tactin® TACS Agarose Strep-Tactin®XT high capacity is a new resin for protein purification via Strep-tag®II or Twin-Strep-tag®. Strep-Tactin®XT high
Presented At: Gibco ExpressionWorld 2018 Presented By: Dennis Niermeier, MS - Scientist, IBA Life Sciences Speaker The video provides a vivid comparison between classical purification process and
Strep-tag affinity chromatography protein purification - theory & practice Column purification of His-tagged recombinant proteins.
Label-free Cell Isolation from PBMCs with Strep-Tactin® Magnetic Microbeads Protein Purification with Magnetic Beads in a 96-well Plate
Affinity chromatography: How to pack columns / cartridges Technology advancements in antibody purification-64762
Magnetic beads are often used for the purification of small amounts of protein in batch. This video shows how our MagStrep Watch the Full Video at Strep-Tactin XT Sepharose
Strep-Tactin®XT technology Strep-Tactin®XT 4Flow® high capacity resin | 4 ml | 2-5030-002
Strep-Tactin® XT Sepharose chromatography resin | Cytiva Strep-tag® protein purification cycle Cleaning of the Wet Fred (Protein Purification Application Aid)
Packing of a Gravity Flow Column for Protein Purification Automated One-Plate Protein Purification with Ni-IMAC IMCStips
Trouble purifying Twin-StrepTagged protein using Strep-Tactin XT Protein Purification: Insights into the Strep-tag® technology how to reuse chromatography columns and nickel resin during protein purification.
The power of the Strep-tag® system goes far beyond standard protein purification. Especially the development of the high affinity This webinar will introduce the Strep-tag® system as a universal platform for the whole protein production and analysis workflow.
Protein Purification with Large Sample Volumes using Wet Fred Binding buffer transfer to a spin column
Mixture transfer so that it can be centrifuged. This webinar describes how new Strep-Tactin Tags can be used for efficient purification of nanobodies produced in mammalian
The structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits This video describes the correct way to remove the detachable end-part of the column before attaching it to the chromatographic The Strep-Tactin®XT 4Flow® high capacity gravity flow columns can be used for the purification of proteins that have been tagged with Strep-tag®II or
Purification: Twin-Strep-Tagged Proteins & Their Complexes On Strep-Tactin Resin l Protocol Preview Strep-Tactin XT Probes| Gator Bio Strep-Tactin® and Strep-Tactin®XT resins for purification of Strep-tagged fusion proteins can be regenerated and re-used 3 to 5
It's easy to set up automated miniaturized protein purifications with Ni-IMAC #IMCStips! To learn more about IMCStips and get a Protein purification is easier than imagined! Let us show you how to purify a protein using batch spin columns and affinity resin. Desalting protein under native conditions using spin columns
Protein Expression: MEXi - IBA's mammalia expression system